This altarpiece was painted by an anonymous Florentine painter, known by his work with Giotto on frescoes in the Upper Church of St. Francis in Assisi, Italy. The design of this piece is similar to the Sienese masters of the late thirteenth-century, the enthroned saint on the center panel with scenes from her story on the left and right sides. Salvaged from the church of St. Cecilia in Florence (which was destroyed by fire in 1304), this alterpiece now resides in the Uffizi Gallery.

The virgin martyr Cecilia is depicted in the center panel, seated on a throne. She wears a black dress, gold-trimmed at the neck and sleeves, enfolded by a red robe, with a white and pink veil trimmed with lace. Cecilia holds a palm frond in her right hand (symbol of martyrdom) and a leather-bound book in her left hand.

The scenes of her life illustated are: (left side) the wedding feast, the conversion of Valerian, an honoring of their chaste marriage, and Cecilia teaching Valerian and brother. (Right side) the baptism of Valerian, Cecilia preaching to crowd, the trial of Cecilia, and her execution attempt in steam bath.